“Protozoan ciliates” are single celled organism. Some marine ciliates have unique features. It is living in the shallow and benthic waters always. It feeds only very minute tiny live organism like cyanobacteria and some algae. It is sometime, infectious nature like diarrhoea occurred. Hence, aseptic nature is very essential one for it culture. It belongs to the order of Hypotrichous. It have many variety of Euplotes species. It have two types of populations. It called as Auto-gamous and Non-autogamos nature. E. crassus is one among the autogamous species. After processing, the researcher took the pure culture under high laboratory condition. We have used molecular procedures to isolate the nuclear materials and sequenced. Further, we have submitted in the NCBI for 18SrRNA.
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